Molecular and antigenic evolution of human influenza A/H3N2 viruses in Quebec, Canada, 2009-2011.
Identifieur interne : 000427 ( Main/Exploration ); précédent : 000426; suivant : 000428Molecular and antigenic evolution of human influenza A/H3N2 viruses in Quebec, Canada, 2009-2011.
Auteurs : Julie Ann [Canada] ; Jesse Papenburg ; Xavier Bouhy ; Chantal Rhéaume ; Marie-Ève Hamelin ; Guy BoivinSource :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [ 1873-5967 ] ; 2012.
Descripteurs français
- KwdFr :
- Antigènes viraux (génétique), Dérive génétique, Glycoprotéine hémagglutinine du virus influenza (génétique), Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains, Mutation, Phylogénie, Québec (épidémiologie), Saisons, Sous-type H3N2 du virus de la grippe A (), Sous-type H3N2 du virus de la grippe A (génétique), Sous-type H3N2 du virus de la grippe A (immunologie), Sous-type H3N2 du virus de la grippe A (isolement et purification), Substitution d'acide aminé, Tests d'inhibition de l'hémagglutination, Tests de neutralisation, Variation des antigènes, Épitopes (génétique), Épitopes (immunologie).
- MESH :
- génétique : Antigènes viraux, Glycoprotéine hémagglutinine du virus influenza, Sous-type H3N2 du virus de la grippe A, Épitopes.
- immunologie : Sous-type H3N2 du virus de la grippe A, Épitopes.
- isolement et purification : Sous-type H3N2 du virus de la grippe A.
- virologie : Grippe humaine.
- épidémiologie : Grippe humaine, Québec.
- Dérive génétique, Humains, Mutation, Phylogénie, Saisons, Sous-type H3N2 du virus de la grippe A, Substitution d'acide aminé, Tests d'inhibition de l'hémagglutination, Tests de neutralisation, Variation des antigènes.
English descriptors
- KwdEn :
- Amino Acid Substitution, Antigenic Variation, Antigens, Viral (genetics), Epitopes (genetics), Epitopes (immunology), Genetic Drift, Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Humans, Influenza A Virus, H3N2 Subtype (classification), Influenza A Virus, H3N2 Subtype (genetics), Influenza A Virus, H3N2 Subtype (immunology), Influenza A Virus, H3N2 Subtype (isolation & purification), Influenza, Human (epidemiology), Influenza, Human (virology), Mutation, Neutralization Tests, Phylogeny, Quebec (epidemiology), Seasons.
- MESH :
- chemical , genetics : Antigens, Viral, Epitopes, Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , immunology : Epitopes.
- geographic , epidemiology : Quebec.
- classification : Influenza A Virus, H3N2 Subtype.
- epidemiology : Influenza, Human.
- genetics : Influenza A Virus, H3N2 Subtype.
- immunology : Influenza A Virus, H3N2 Subtype.
- isolation & purification : Influenza A Virus, H3N2 Subtype.
- virology : Influenza, Human.
- Amino Acid Substitution, Antigenic Variation, Genetic Drift, Hemagglutination Inhibition Tests, Humans, Mutation, Neutralization Tests, Phylogeny, Seasons.
Abstract
BACKGROUND
A/H3N2 variability leads to poor vaccine effectiveness when the vaccine strain is not well matched to the circulating virus.
OBJECTIVES
We aim to describe the molecular and antigenic evolution of A/H3N2 viruses recovered during the last 3 influenza seasons in Quebec, Canada.
STUDY DESIGN
Clinical samples from 33 patients with culture-confirmed A/H3N2 infections were collected over 3 consecutive seasons (March 2009-2011). The isolates' HA gene was amplified and sequenced; phylogenetic analyses of the HA1 region were conducted. To characterize A/H3N2 antigenic properties, standard hemagglutination inhibition (HI) and microneutralization (MN) assays were performed.
RESULTS
In 2009, we observed an antigenic drift from A/Brisbane/10/2007 (vaccine strain used in 2008-2009 and 2009-2010) to A/Perth/16/2009 (vaccine strain used in 2010-2011). Antigenic analysis of clinical influenza strains recovered in Quebec during 2009-2010 also illustrated antigenic drift from the previously prevalent A/Brisbane/10/2007-like (March 2009) to A/Perth/16/2009-like (December 2009) strains. In 2010-2011, the emergence of >4 substitutions in 4 different H3 antigenic sites suggested a genetic drift. However, HI and MN results confirmed the emergence of a drift in only 1 strain (8-fold difference in titers), while 19 others remained antigenically similar to A/Perth/16/2009 but exhibited titer differences (2-4-fold) just inferior to the standard definition of a drift.
CONCLUSION
Antigenic and molecular characterization of H3N2 viruses over three seasons revealed that not only is the number of HA mutations important, but the nature and location of key mutations may play a significant role in antigenic drift.
DOI: 10.1016/j.jcv.2011.09.016
PubMed: 22036039
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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l'hémagglutination</term><term>Tests de neutralisation</term><term>Variation des antigènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>A/H3N2 variability leads to poor vaccine effectiveness when the vaccine strain is not well matched to the circulating virus.</p></div><div type="abstract" xml:lang="en"><p><b>OBJECTIVES</b></p><p>We aim to describe the molecular and antigenic evolution of A/H3N2 viruses recovered during the last 3 influenza seasons in Quebec, Canada.</p></div><div type="abstract" xml:lang="en"><p><b>STUDY DESIGN</b></p><p>Clinical samples from 33 patients with culture-confirmed A/H3N2 infections were collected over 3 consecutive seasons (March 2009-2011). The isolates' HA gene was amplified and sequenced; phylogenetic analyses of the HA1 region were conducted. To characterize A/H3N2 antigenic properties, standard hemagglutination inhibition (HI) and microneutralization (MN) assays were performed.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In 2009, we observed an antigenic drift from A/Brisbane/10/2007 (vaccine strain used in 2008-2009 and 2009-2010) to A/Perth/16/2009 (vaccine strain used in 2010-2011). Antigenic analysis of clinical influenza strains recovered in Quebec during 2009-2010 also illustrated antigenic drift from the previously prevalent A/Brisbane/10/2007-like (March 2009) to A/Perth/16/2009-like (December 2009) strains. In 2010-2011, the emergence of >4 substitutions in 4 different H3 antigenic sites suggested a genetic drift. However, HI and MN results confirmed the emergence of a drift in only 1 strain (8-fold difference in titers), while 19 others remained antigenically similar to A/Perth/16/2009 but exhibited titer differences (2-4-fold) just inferior to the standard definition of a drift.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>Antigenic and molecular characterization of H3N2 viruses over three seasons revealed that not only is the number of HA mutations important, but the nature and location of key mutations may play a significant role in antigenic drift.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22036039</PMID><DateCompleted><Year>2012</Year><Month>03</Month><Day>26</Day></DateCompleted><DateRevised><Year>2011</Year><Month>12</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-5967</ISSN><JournalIssue CitedMedium="Internet"><Volume>53</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology</Title><ISOAbbreviation>J. Clin. Virol.</ISOAbbreviation></Journal><ArticleTitle>Molecular and antigenic evolution of human influenza A/H3N2 viruses in Quebec, Canada, 2009-2011.</ArticleTitle><Pagination><MedlinePgn>88-92</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jcv.2011.09.016</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">A/H3N2 variability leads to poor vaccine effectiveness when the vaccine strain is not well matched to the circulating virus.</AbstractText><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">We aim to describe the molecular and antigenic evolution of A/H3N2 viruses recovered during the last 3 influenza seasons in Quebec, Canada.</AbstractText><AbstractText Label="STUDY DESIGN" NlmCategory="METHODS">Clinical samples from 33 patients with culture-confirmed A/H3N2 infections were collected over 3 consecutive seasons (March 2009-2011). The isolates' HA gene was amplified and sequenced; phylogenetic analyses of the HA1 region were conducted. To characterize A/H3N2 antigenic properties, standard hemagglutination inhibition (HI) and microneutralization (MN) assays were performed.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In 2009, we observed an antigenic drift from A/Brisbane/10/2007 (vaccine strain used in 2008-2009 and 2009-2010) to A/Perth/16/2009 (vaccine strain used in 2010-2011). Antigenic analysis of clinical influenza strains recovered in Quebec during 2009-2010 also illustrated antigenic drift from the previously prevalent A/Brisbane/10/2007-like (March 2009) to A/Perth/16/2009-like (December 2009) strains. In 2010-2011, the emergence of >4 substitutions in 4 different H3 antigenic sites suggested a genetic drift. However, HI and MN results confirmed the emergence of a drift in only 1 strain (8-fold difference in titers), while 19 others remained antigenically similar to A/Perth/16/2009 but exhibited titer differences (2-4-fold) just inferior to the standard definition of a drift.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Antigenic and molecular characterization of H3N2 viruses over three seasons revealed that not only is the number of HA mutations important, but the nature and location of key mutations may play a significant role in antigenic drift.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ann</LastName><ForeName>Julie</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Research Center in Infectious Diseases of the CHUQ-CHUL and Laval University, Québec, Québec, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Papenburg</LastName><ForeName>Jesse</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Bouhy</LastName><ForeName>Xavier</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Rhéaume</LastName><ForeName>Chantal</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Hamelin</LastName><ForeName>Marie-Ève</ForeName><Initials>MÈ</Initials></Author><Author ValidYN="Y"><LastName>Boivin</LastName><ForeName>Guy</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>10</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Clin Virol</MedlineTA><NlmUniqueID>9815671</NlmUniqueID><ISSNLinking>1386-6532</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019943" MajorTopicYN="N">Amino Acid Substitution</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000940" MajorTopicYN="Y">Antigenic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040961" MajorTopicYN="Y">Genetic Drift</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006385" MajorTopicYN="N">Hemagglutination Inhibition Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053122" MajorTopicYN="N">Influenza A Virus, H3N2 Subtype</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" 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PubStatus="entrez"><Year>2011</Year><Month>11</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>11</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22036039</ArticleId><ArticleId IdType="pii">S1386-6532(11)00379-9</ArticleId><ArticleId IdType="doi">10.1016/j.jcv.2011.09.016</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Boivin, Guy" sort="Boivin, Guy" uniqKey="Boivin G" first="Guy" last="Boivin">Guy Boivin</name><name sortKey="Bouhy, Xavier" sort="Bouhy, Xavier" uniqKey="Bouhy X" first="Xavier" last="Bouhy">Xavier Bouhy</name><name sortKey="Hamelin, Marie Eve" sort="Hamelin, Marie Eve" uniqKey="Hamelin M" first="Marie-Ève" last="Hamelin">Marie-Ève Hamelin</name><name sortKey="Papenburg, Jesse" sort="Papenburg, Jesse" uniqKey="Papenburg J" first="Jesse" last="Papenburg">Jesse Papenburg</name><name sortKey="Rheaume, Chantal" sort="Rheaume, Chantal" uniqKey="Rheaume C" first="Chantal" last="Rhéaume">Chantal Rhéaume</name></noCountry><country name="Canada"><noRegion><name sortKey="Ann, Julie" sort="Ann, Julie" uniqKey="Ann J" first="Julie" last="Ann">Julie Ann</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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